Erma Safitri, - and Suzanita Utama, - and Candra Bumi, - and Sri Wigati Mardi Mulyani, - and Endang Retnowati, - and Purwati, - and R. Heru Prasetyo, - and Mas'ud Hariadi, - and Aulani’am, - and Ferdiansyah Mahyudin, - and Fedik Abdul Rantam, - (2014) Hypoxic Preconditioning For Viable and Self Renewing Mesenchymal Stem Cells (Mscs) As The Regeneration of Spermatogenesis Process. Journal of Applied Sciences Research, 8 (8). pp. 42-46. ISSN 1816-157X e-ISSN 1819-544X

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Abstract

The aim of this research was to obtain MSCs that were viable and self renewing for spermatogenesis process by a treatment of hypoxic precondition in vitro culture. In this research, hypoxic precondition was the use of 1% O2 concentration which was compared to those of culture under normoxic (21% O2) condition. Flowcytometric analysis showed that in MSCs culture under 1% O2 concentration, the level of CD90+ and CD34- were not altered (remained undifferentiated), meanwhile under 21% O2 concentration, cells have experienced alteration (became differentiated), that was indicated by the down regulation of CD90+ and up regulation of CD34-. Immunocytochemical and immunofluorescence analysis showed that under 1% O2 concentration, MSCs culture expressed transcription factors, such as OCT4 and SOX2, meanwhile under 21% O2 concentration, the transcription factors OCT4 and SOX2 (self renewal function), were not expressed. In conclusion, this research showed that hypoxic preconditioning with 1% O2 concentration very supported MSCs to remain viable before transplantation for spermatogenesis disorder, because the cells still undifferentiated and self renewal capacity was maintained.

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