OKKY HERMAWAN, ,- and Woro Hastuti Satyantini, ,- and Prayogo, ,- (2016) EFEK PENAMBAHAN KITOSAN TERHADAP PERUBAHAN JUMLAH TOTAL HEMOSIT DAN DAYA TAHAN TERHADAP STRES SALINITAS PADA UDANG VANAME (Litopenaeus vannamei). Journal of Aquaculture and Fish Health, 5 (3). pp. 100-107. ISSN 2301-7309

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	Text (Artikel) Artikel 24. EFEK PENAMBAHAN KITOSAN TERHADAP PERUBAHAN JUMLAH TOTAL HEMOSIT DAN DAYA TAHAN TERHADAP STRES SALINITAS PADA UDANG VANAME (Litopenaeus vannamei) (1).pdf Download (806kB)
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Abstract

White shrimp is a one of leading commodity fisheries industry in Indonesia. There are some constraints in its culture, caused by viruses and bacteria. But it can be solved by increasing the immune system of shrimp. Chitosan is one of the materials that can be used as an immunostimulant. Chitosan can stimulate the immune system. Stress is a physiological response that occurs when the animals try to maintain condition of environmental changed. The environmental factor such as temperature, dissolved oxygen and salinity may change total haemocyte count. Total haemocyte count can be used to know the health status of the shrimp. The purpose of research to determine the effect of addition chitosan on feed to total haemocyte count changes and salinity stress tolerance. The method of research used experimentaly method by using completely random design (RAL) with four treatments and four repetitions. Treatments which were used addition chitosan 0 mg/kg diet (A1), 250 mg/kg diet (A2), 500 mg/kg diet (A3) and 1000 mg/kg diet (A4). The main parameter which was observed were total haemocyte count changes for 14 days, 24 hours and 48 hours after salinity stress test, survival rate at salinity stress test and water quality. The results were analyzed by Analysis of Varian (Anova) continued by Duncan’s Multiple Range Test. The result showed that chitosan addition in feed for 14 days and 24 hours after salinity stress test, gave the significantly different (P<0.05) to total haemocyte count of white shrimp with the highest of total haemocyte count obtained in treatment of A2. Fourty eight hours after salinity stress test did not give the significant different to total haemocyte count and survival rate of white shrimp

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