Dian Agustin Wahjuningrum, - and Setyabudi, - and Fauzan, - and Mirza Bahar Firnanda, - and Devi Eka Juniarti, - and Purwati, - and Fery Setiawan, - and Anuj Bhardwaj, - and Ajinkya M. Pawar, - (2022) Osteogenesis Effect of Human Adipose-derived Mesenchymal Stem Cell with Chitosan Scaffold in White Rat (Rattus norvegicus) Bone Defect on Serum Alkaline Phosphatase. Journal of International Dental and Medical Research.

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Abstract

Bone defect is one of the challenges for dentists in the process of healing bone tissue. Bone defect can occur in alveolar bone with the etiology of microorganisms and cyst expansion. In addition, cases of bone defects in alveolar bone are also often found in cases with treatment of apex resection and hemisection. Autologous bone graft is a clinical gold standard in the treatment of bone defect. However, the use of bone graft has a limited number of growth factors produced. Tissue engineering is the latest method in terms of bone regeneration. Tissue engineering has three main components; stem cell, growth factor, and scaffold. Stem cells will increase osteoblastogenesis and chitosan scaffold will immobilize alkaline phosphatase (ALP) so that serum ALP levels decrease and bone regeneration and mineralization processes become faster. The aim of this study is to analyze osteogenesis effect of human adipose-derived mesenchymal stem cell (HADMSC) with chitosan scaffold (CS) in white rat bone defect on serum alkaline phosphatase (ALP) levels. This research was a in vivo laboratory experimental study. Bone defects are planted with chitosan scaffold (CS) and a combination of human adipose-derived mesenchymal stem cells (HADMSC) with chitosan scaffold. Measurement of ALP levels was carried out by the International Federation of Clinical Chemistry (IFCC) method using an analyzer on the 1st, 3rd, 7th and 14th days. Research data were analyzed using multivariate analysis of variance (MANOVA) and Bonferroni tests. The results of the data analysis showed that there were significant differences in ALP levels with CS planting and the combination of HADMSC and CS. Osteogenesis effect of human adiposederived mesenchymal stem cell (HADMSC) with chitosan scaffold (CS) in white rat bone defect reduces serum alkaline phosphatase (ALP) levels on the 3rd and 14th days

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