Veronica Regina Rosselle, - and Cendranata Wibawa Ongkowijoyo, - and Setyabudi, - and Sri Kunarti, - and Nirawati Pribadi, - (2022) Comparison of Enterococcus faecalis biofilm degradation after diode laser exposure with Chlorophyll and Psoralens photosensitizer. Bali Medical Journal (BaliMedJ).

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Abstract

Introduction: Enterococcus faecalis are dominant in root canal infection and can survive harsh conditions such as high pH, scarce nutrients, and heat. They form mature biofilm readily and can resist disinfection approaches. Photo-activated disinfection with photosensitizer and laser irradiation is a viable alternative to eliminate E. faecalis biofilm. This study aimed to investigate and compare E. Faecalis biofilm degradation using photoactivated disinfection with chlorophyll or psoralens photosensitizer and 405 nm diode laser irradiation. Methods: This study used E. Faecalis biofilm grown in the laboratory. Chlorophyll or psoralens were applied as a photosensitizer, and then 405 nm diode laser was irradiated for 30 or 60 seconds. The effectiveness of biofilm degradation was measured with optical density using microplate reader. Results: Chlorophyll with 30 seconds of laser irradiation results in 30.8% biofilm degradation, whereas psoralens with 60 seconds of laser irradiation result in 81.8% biofilm degradation. One-way ANOVA test results in significant difference in biofilm degradation effectiveness amongst all groups. Conclusion: Psoralens with 60 seconds of 405nm diode laser irradiation yielded the most Enterococcus faecalis biofilm degradation compared to other groups

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