Lipopolysaccharide’s Cytotoxicity on Human Umbilical Cord Mesenchymal Stem Cells

Mefina Kuntjoro, - and Eric Priyo Prasetyo, - and Febriastuti Cahyani, - and Michael Josef Kridanto Kamadjaja, - and Nike Hendrijantini, - and Harry Laksono, - and Primanda Nur Rahmania, - and Vivin Ariestania, - and Alexander Patera Nugraha, - and Igo Syaiful Ihsan, - and Aristika Dinaryanti, - and Fedik Abdul Rantam, - (2020) Lipopolysaccharide’s Cytotoxicity on Human Umbilical Cord Mesenchymal Stem Cells. Pesquisa Brasileira em Odontopediatria e Clínica Integrada, 20. pp. 1-7. ISSN 1983-4632

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Objective: To show the cytotoxicity of Porphyromonas gingivalis lipopolysaccharide (LPS) on human umbilical cord mesenchymal stem cells (HUCMSCs) to better understand the characteristics for its application in regenerative procedures under periodontopathogen LPS influence. Material and Methods: Ultrapure Porphyromonas gingivalis LPS was used in this study. This research used a frozen stock HUCMSCs, previously confirmed by flow cytometry. The biological characteristics, such as cell morphology, proliferation, and protein expression, were screened. To check the cytotoxicity, HUCMSCs were cultured and divided into two groups, the control group and LPS group with various concentrations from 25 to 0.39 μg/mL. MTT assay was done and the cells were observed and counted. The significance level was set at 5%. Results: The percentage of living HUCMSCs on LPS group were not significantly different among concentrations (p>0.05) from 25 to 0.39 μg/mL, even though there were slight mean decrease between groups, but they were not significant. The duration of 24 hours of exposure of LPS does not significantly lower HUCMSCs viability. Conclusion: LPS does not affect the viability of HUCMSCs. The lower the concentration of LPS, the higher the viability of HUCMSCs.

Item Type: Article
Subjects: R Medicine
R Medicine > RK Dentistry
R Medicine > RK Dentistry > RK641-667 Prosthetic dentistry. Prosthodontics
Divisions: 02. Fakultas Kedokteran Gigi > Prosthodontics
Mefina Kuntjoro, -NIDN0029097905
Eric Priyo Prasetyo, --
Febriastuti Cahyani, -NIDN0005027909
Michael Josef Kridanto Kamadjaja, -NIDN0031056301
Nike Hendrijantini, -NIDN0006105908
Harry Laksono, --
Primanda Nur Rahmania, --
Vivin Ariestania, --
Alexander Patera Nugraha, --
Igo Syaiful Ihsan, --
Aristika Dinaryanti, --
Fedik Abdul Rantam, -NIDN0010035907
Depositing User: Rudy Febiyanto
Date Deposited: 16 Apr 2023 08:09
Last Modified: 05 Jun 2023 06:51
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