Maretaningtias Dwi Ariani, - and Pramita Putri Larasati, - and Hanoem Eka Hidajati, - and Wahjuni Widajati, - and Utari Kresnoadi, - (2017) In vitro evaluation of FGF-2 on osteoblast cell seeded in nano chitosancarbonate apatite chitosan scaffold. In Proceedings of the 7th International Meeting and the 4th Joint Scientific Meeting in Dentistry-TIMNAS7-JSMID4.

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Abstract

Background: Scaffold is a medium for extracellular matrix synthesis and Becomes a place for attachment and growth of new cells. Some polymeric materials, the which have been developed in tissue engineering are chitosan. Chitosan is one of the best materials used in tissue engineering due to its biodegradable, biocompability, anti-bacterial properties, for wound healing and bioadded characters. The carbonate apatite material is used as a biocompatible material for medical purposes. Carbonate apatite Increased material the chemical bonds between biomaterials and bone tissue that can Affect bone formation. The nanostructured biomaterials Significantly Enhance cell functioning leading to Increased osteoinductivity and oseointegrativity. So based on the explanation and understanding of the mechanisms of the relationship between the bone regeneration process and the nano chitosancarbonate apatite scaffold, it is important to improve the method for the next generation of scaffolds that can demonstrate bone efficiency and regeneration in vitro. Objective: To Determine the Increased expression of FGF-2 on osteoblast cell planting in nano chitosancarbonate apatite scaffold and the results of this study are expected to be used as a selection method of bone graft using nano-chitosan scaffold. Methods: Laboratory experimental study with post test only group design on seven samples, the osteoblast cells planted on chitosan nano-carbonate apatite scaffold with observation time on day 3, day 5, and day 7 and Increased expression of FGF- 2 osteoblast cells were measured by Immunohistochemistry (IHC) method. The results were Analyzed by statistical test of multiple comparison test of Post Hoc and Tukey test. Result: The results Showed a significant difference (P <0.05) between treatment groups in the observation time on day 3, 5 and 7. Conclusion: The experiment has shown that the increase is of FGF-2 expression in osteoblast cells planted in chitosan nanocarbonate apatite scaffold has Increased in observation time on day 3, 5 and 7.

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