Widya Saraswati, - and Nina Dhaniar, - and Hermawan Adi Praja, - and Yovita Yonas, - and Saindra Arsa Gumilang, - and Yansha Mutia Dyah Kusumastuti, - (2020) INVOLVEMENT OF THE NLRP3 IN ACTIVATING THE IMMUNE RESPONSE AFTER RESIN HEMA EXPOSURE IN DENTINE-PULP COMPLEX. Biochem. Cell. Arch, 20 (2).

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Abstract

This study aimed to investigate the involvement of the NLRP3 in activating the immune response after resin monomer 2-hydroxyethyl methacrylate (HEMA) exposure in dentine-pulp complex. The research samples were 28 male Sprague Daley rats in randomized post-test only control groups. The rats were randomly divided into 4 groups, with 7 rats per group. Group 1, the control group was treated with glass ionomer cement restoration (Fuji IX LC) without HEMA application after the lowermolarwas drilled using a low speed round bur. Group 2-4, the experimental groups were treated with HEMAliquid (Sigma Aldrich) and sealed with glass ionomer cement restoration afterthe lower molar was drilled using a low speed round bur, and the teeth in each group were extracted after24, 48 and 72 hours accordingly. The teeth were soaked into EDTAfor 8 weeks, then cut 5milimicron by microtome then made the paraffin block. Immunohistochemistry staining was smeared using anti NLRP3 antibody monoclonal. The samples were then statistically analyzed using ANOVAand Tukey HSD. The NLRP3 expression of odontoblast reached the highest number at 48 hours of experimental time. NLRP3 has a role in activating the immune response after resin HEMA exposure in dentine-pulp complex.

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