Kun Ismiyatin, - and LeidyHerlin Rumbiak, - and Widya Saraswati, - and Sri Kunarti, - and Anuj Bhardwaj, - (2019) Effect of 405 nm Diode Laser with Varying Irradiation Time on BHK-21 Fibroblast Viability. Conservative Dentistry Journal, 9 (1).

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Abstract

Background:Laser isa device that emits light through a process of optical amplification based on the stimulated emission of electromagnetic radiation; it can beused for surgery, detoxification, bio stimulationandantibacterial. However, lasers have Biphasic Dose Response (BDR), which is bio stimulationand bio inhibition. To determine if405 nm laser diode is biocompatible, viability test is necessary before these lasers can belabeled as viable to use in dental therapy. Aim: To prove the variation of radiation time of the 405 nm laser diode radiation can cause bio stimulationand bioinhibitionresponse that affects the viability of BHK-21 fibroblast cells. Method: Viability test was carried out using BHK-21 fibroblast cells which were inserted into 96-well microplate, then radiated with 405 nm laser diode with varyingirradiation time of 30s, 60s, 120s, 240s and 480s. After radiation, the cellsare then incubated for 24h. Cytotoxicity was observed using MTT assay andELISA reader. Data was analyzed using the Kolmogorov-Smirnov test, Levene Test, Welch ANOVA, and Tukey HSD. Results: BHK-21 fibroblast cells radiated with 405 nm laser diode with radiation timeof 30s, 60s, 120s, and 240shave the same viability as the control cell, while at 480 seconds the viability exceeds that of the control cell. Conclusion: 405 nm laser diode with radiation times of 30s, 60s, 120s, and 240sdo not affect the viability of BHK-21fibroblast cells. Meanwhile, 480s irradiation time of 405 nm laser diode causes biostimulationresponse that increases the viability of BHK-21 fibroblast cells

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