Akhmad Taufiq Mukti, ,- (2017) METODE PENENTUAN PLOIDI PADA IKAN NILA OREOCHROMIS NILOTICUS MENGGUNAKAN KOMBINASI SEBARAN KROMOSOM DAN NUKLEOLI SECARA BERSAMAAN. Jurnal Akuakultur Sungai dan Danau, 2 (1). pp. 43-50. ISSN 2503-4766

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Abstract

Ploidy determination in fish was need the accurate, simple and rapid method. This research was aimed to develop ploidy determination method in Nile tilapia use combination of chromosome and nucleoli spreads simultaneously. Ten fish of 30-day-old Nile tilapias were used in this research. Solid tissues for preparation were collected on the caudal fin of the fish. The result showed that chromosome and nucleoli spreads simultaneously were easily observed in same preparate. Ploidy determination in Nile tilapia can done very accurate and fast use combination of chromosome and nucleoli spreads simultaneously as indicators to ploidy level determination in fish. Keywords: chromosome spread, nucleoli spread, simultaneously, ploidy level, tilapia

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