Suryo Prasetyo, Suryo and Furqan Hidayatullah, Furqan and Indra Bachtiar, Indra and Arif Rachman, Arif and Indri Lakhsmi Putri, Indri and Fabio Castiglione, Fabio and Doddy M. Soebadi, Doddy and Lukman Hakim, Lukman The Effect Of Conditioned Medium Adipose Derived Mesenchymal Stem Cells In Urothelial Carcinoma Culture Cells Viability. Indonesian Journal of Urology, 28 (1). ISSN p(0853-442X) e(2355-1402)

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Abstract

Abstract Objective: This study aimed to gives a perspective in CM-ADMSCs effect in urothelial bladder cancer viability. Material & Methods: Human bladder cell carcinoma type 5637 was used as the subject of this in vitro study. This study contains four different groups: untreated control group, Culture medium: hADMSCs with 1:1, 1:2, and 1:4 concentration group. Each group consists of 6 replications to prevent bias of the study. Viability was determined with MTT assay methods and evaluation performed after 48 h exposure of conditioned medium. Results: A post hoc test was conducted to analyze the data. The 5637 bladder cancer cell line demonstrated significantly decreased viability after exposure to culture medium: CM-hADMSCs 1:1 (p: 0.002) compared to the negative control group, but there are no significant differences in viability between the control groups with groups that were exposed to culture medium: CM-hADMSCs 1:2 and culture medium: CM-hADMSCs 1:4 with p: 0.480 and p: 0.060 respectively. Conclusion: Decreased viability of urothelial bladder cancer cells after exposure to CM-hADMSCs occurs at a concentration of 1:1 and Dosage addition more than 1:1 concentration doesn’t give any advantages.

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