Hypoxia Effects in Intervertebral Disc-Derived Stem Cells and Discus Secretomes: An in vitro Study

Romaniyanto, - and Ferdiansyah Mahyudin, - and Cita Rosita Sigit Prakoeswa, - and Hari Basuki Notobroto, - and Damayanti Tinduh, - and Ryan Ausrin, - and Fedik Abdul Rantam, - and Heri Suroto, - and Dwikora Novembri Utomo, - and Sholahuddin Rhatomy, - (2022) Hypoxia Effects in Intervertebral Disc-Derived Stem Cells and Discus Secretomes: An in vitro Study. Stem Cells and Cloning: Advances and Applications, 15. pp. 21-28. ISSN 1178-6957

[img] Text (Artikel)
C11_Artikel.pdf

Download (2MB)
[img] Text (Turnitin)
C11_Turnitin.pdf

Download (3MB)
[img] Text (Validasi)
C11_Validasi.pdf

Download (484kB)
Official URL: https://www.dovepress.com/index.php

Abstract

Background: This study aimed to investigate the effects of hypoxia and normoxia preconditioning in rabbit intervertebral disc-derived stem cells (IVDSCs) and discus-derived conditioned medium (DD-CM)/secretomes in vitro. Transforming growth factor (TGF)-β1, platelet-derived growth factor (PDGF), fibroblast growth factor (FGF), and vascular endothelial growth factor (VEGF) have a role in the proliferation, development, differentiation, and migration of MSCs. Materials and methods: Intervertebral discs were isolated from rabbit and incubated in normoxia and hypoxia 1%, 3%, and 5% (hypoxia groups) condition. Cell counting was performed after 24 hours of manipulation, then analyzed using one-way ANOVA. TGF-β1, PDGF, FGF, and VEGF were measured using the ELISA. Results: The highest number of cells was in the hypoxia 3% preconditioning compared to the normoxia, hypoxia 1%, and hypoxia 5% groups. Hypoxia 3% also had the highest increase in PDGF protein production compared to normoxia, with hypoxia 1% and 5%. Among hypoxia groups, the highest secretions of VEGF and FGF proteins were in the hypoxia 3% group. Based on TGF-β1 protein measurement, the hypoxia 1% group was the highest increase in this protein compared to other groups. Conclusion: Oxygen level in hypoxia preconditioning has a role in the preparation of IVDSCs and secretome preparation in vitro. The highest cell numbers were found in the treatment group with 3% hypoxia, and 3% hypoxia was significantly related to support IVDSCs preparation. Preconditioning with 3% hypoxia had higher PDGF and VEGF levels than other hypoxia groups.

Item Type: Article
Subjects: R Medicine
Divisions: 10. Fakultas Kesehatan Masyarakat > Biostatistika dan Kependudukan
Creators:
CreatorsNIM
Romaniyanto, -UNSPECIFIED
Ferdiansyah Mahyudin, -NIDN8890010016
Cita Rosita Sigit Prakoeswa, -NIDN8865610016
Hari Basuki Notobroto, -NIDN0025066504
Damayanti Tinduh, -NIDN8889900016
Ryan Ausrin, --
Fedik Abdul Rantam, -NIDN0010035907
Heri Suroto, -NIDN8802630017
Dwikora Novembri Utomo, -NIDN8897700016
Sholahuddin Rhatomy, -UNSPECIFIED
Depositing User: Tn Chusnul Chuluq
Date Deposited: 27 Apr 2023 00:55
Last Modified: 27 Apr 2023 00:55
URI: http://repository.unair.ac.id/id/eprint/124840
Sosial Share:

Actions (login required)

View Item View Item