Assessment of fish biodiversity in four Korean rivers using environmental DNA metabarcoding

Md Jobaidul Alam, ,- and Nack-Keun Kim, ,- and Sapto Andriyono, ,- and Hee-kyu Choi, ,- and Ji-Hyun Lee, ,- and Hyun-Woo Kim, ,- (2016) Assessment of fish biodiversity in four Korean rivers using environmental DNA metabarcoding. Peer Journal - Biodiversity and Conservation, 5 (3). ISSN 2167-8359

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Environmental DNA (eDNA) metabarcoding is a cost-effective novel approach to estimate biodiversity in an ecosystem. In this study, the MiFish pipeline was employed to test if the system methodology is sufficiently reliable to estimate fish biodiversity in Korean rivers. A total of 125 unique haplotypes and 73 species were identified at the species level from 16 water samples collected from a single survey in four Korean rivers (Hyeongsan, Taehwa, Seomjin, and Nakdong). Among the four rivers, the highest species richness was recorded in the Seomjin River (52 species), followed by the Taehwa (42 species) and Hyeongsan (40 species) rivers. The Nakdong River (26 species) presented the lowest species richness and number of endemic species, presumably due to its metropolitan location and anthropogenic impacts, such as dams or weirs. We were also able to detect that five exotic species (Carassius cuvieri, Cyprinus carpio, Cyprinus megalophthalmus, Lepomis macrochirus, and Micropterus salmoides) are widely distributed in all surveyed rivers, a situation that might be problematic in terms of conservation. Our findings indicate that the eDNA metabarcoding technique is one of the most cost-effective scientific tools available for the management and conservation of the freshwater fish resources available in Korea. However, the low number of 12S sequences of endemic species in the database and low resolution of the MiFish region for differentiating several taxa should be upgraded for their wide use.

Item Type: Article
Uncontrolled Keywords: Biodiversity, Korea, Next-generation sequencing, MiFish pipeline, eDNA metabarcoding
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
S Agriculture > SH Aquaculture. Fisheries. Angling > SH1-691 Aquaculture. Fisheries. Angling > SH20.3-191 Aquaculture
Divisions: 14. Fakultas Perikanan dan Kelautan > Kelautan
Md Jobaidul Alam, ,-UNSPECIFIED
Nack-Keun Kim, ,-UNSPECIFIED
Sapto Andriyono, ,-0025097907
Hee-kyu Choi, ,-UNSPECIFIED
Depositing User: anita erna faricha
Date Deposited: 28 Apr 2023 09:30
Last Modified: 20 Jun 2023 02:01
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