Production of the secondary metabolite catechin by in vitro cultures of Camellia sinensis L

Sutini, - and Widiwurjani, - and Chrismawan Ardianto, - and Junaidi Khotib, - and Djoko Agus Purwanto, - and Wirdhatul Muslihatin, - (2020) Production of the secondary metabolite catechin by in vitro cultures of Camellia sinensis L. Journal of Basic and Clinical Physiology and Pharmacology, 31 (5). pp. 1-7. ISSN 0792-6855

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Official URL: https://www.degruyter.com/document/doi/10.1515/jbc...

Abstract

Background Catechin is one of the secondary metabolites in Camellia sinensis L. that is alternatively produced through in vitro cultures. The in vitro culture product is possibly improved by optimizing the culture medium with the addition of growth regulators and precursors. The purpose of this study was to confirm the success of the secondary catechin metabolite production through the in vitro culture of C. sinensis L in a relatively short time. Methods The secondary catechin metabolite product is obtained in about 40 days. The study was conducted by (1) leaf cutting for inoculation in Murashige and Skoog media with 1 μg/mL of 2,4-dichlorophenoxyacetic acid growth regulator; (2) the inoculation of callus multiplication on the same medium as a partially modified inoculation media condition with the addition of 1 μg/mL of 6-benzylaminopurine (BAP) and 2 μg/mL of 2,4-dichlorophenoxyacetic acid at concentration; (3) callus multiplication developed on a new medium containing phenylalanine precursors (300 μg/mL); (4) testing growth by harvesting the callus and weighing the wet weight of its biomass and (5) identification of the callus qualitatively and quantitatively by using high-performance liquid chromatography (HPLC). Results The level of secondary catechin metabolite produced was 2.54 μg/mL and 12.13 μg/mL in solid and suspension media, respectively. Conclusions It is concluded that the method is effective and efficient in producing catechin product from C. sinensis L.

Item Type: Article
Uncontrolled Keywords: Camellia sinensis L; catechin; growth regulator; in vitro culture; phenylalanine; secondary metabolite
Subjects: R Medicine
R Medicine > RS Pharmacy and materia medica
R Medicine > RS Pharmacy and materia medica > RS1-441 Pharmacy and materia medica
R Medicine > RS Pharmacy and materia medica > RS200-201 Pharmaceutical dosage forms
Divisions: 05. Fakultas Farmasi > Farmasi Klinis
Creators:
CreatorsNIM
Sutini, --
Widiwurjani, --
Chrismawan Ardianto, -NIDN0029028403
Junaidi Khotib, -NIDN0022107001
Djoko Agus Purwanto, -NIDN0008055906
Wirdhatul Muslihatin, --
Depositing User: Mr M. Fuad Sofyan
Date Deposited: 28 Apr 2023 01:22
Last Modified: 28 Apr 2023 01:22
URI: http://repository.unair.ac.id/id/eprint/125195
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