Lusiana Arifianti, - and Sukardiman, - and Niken Indriyanti, - and Retno Widyowati, - (2020) Anticancer property of Orthosiphon stamineus Benth. extracts in different solvent systems against T47D Human Breast Cancer cell lines. Fabad Journal of Pharmaceutical Sciences, 45 (3). pp. 187-194. ISSN 1300-4182

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Abstract

Solvent system is an important factor in extraction process in order to obtain compounds that have pharmacological activity. The aim of this research is to develop a comprehensive extraction methods by modification of solvents used that might produce compounds possessing pharmacological activity for anticancer. In this study, Orthosiphon stamineus Benth. extract was used as sample in different solvent systems to observe their metabolite profiles. Extraction carried out using sonication techniques with ethanol solvents in three types of concentrations (96%, 70% and 50%). Then, the extracts introduced into anticancer activity profiles in order to find its active compounds. The anticancer activity had explored against breast cancer cells (T47D) using the MTT assay and doxorubicin as a positive control. The best IC50 value obtained from the 50% ethanol extract of Orthosiphon stamineus Benth. Based on the scanning chromatogram at 366 nm using Thin Layer Chromatography, each sample contains sinensetin and rosmarinic acid. The largest percentage of rosmarinic acid area was found on 70% ethanol extract of Orthosiphon stamineus Benth, while the highest percentage of sinensetin was found on 50% ethanol extract of Orthosiphon stamineus Benth. Thus, it can be concluded that sinensetin which has the most influence on anticancer activity.

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