Sri Sumarsih*,, .- and Ni’matuzahroh, .- and Fatimah,, .- and Miranti Puspitasari,, .- and Meilisa Rusdiana, .- (2017) Effect Of Aliphatic And Aromatic Hydrocarbons On The Oxygenase Production From Hydrocarbonoclastic Bacteria. Journal of Chemical Technology and Metallurgy, 52 (6). pp. 1062-1069. ISSN ISSN 1314-7471 (print) ISSN 1314-7978 (on line)

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Abstract

This research aims to study the effect of aliphatic and aromatic hydrocarbon on oxygenase production from four hydrocarbonoclastic bacteria, including Actinobacillus sp. P3(7), Bacillus subtilis 3KP, Micrococcus sp. LII(61), and Pseudomonas putida T1(8). The bacterial growth during cultivation in a mineral salt medium, containing 1 % hydrocarbons (hexadecane, toluene, and naphthalene) was observed by measuring the optical density of the culture medium at λ 600 nm. The activity of the intracellular catabolic enzyme was determined by measuring the change of NADH absorbance at λ = 340 nm with an UV/Vis spectrophotometer. The results showed that the addition of aliphatic and aromatic hydrocarbons in the growth media enhanced the growth and production of oxygenase from the four hydrocarbonoclastic bacteria. The addition of 1 % hexadecane and 1 % naphthalene into cell culture of Pseudomonas putidaT1(8) increased the oxygenase activity up to 8.789 times, and 20.770 times, respectivelly. The addition of 1 % toluene into cell culture of Micrococcus sp. LII(61) increased the oxygenase activity up to 15.72 times

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