Fatimah, -. and Silvia Kurnia Sari,, .- and Nastiti Trikurniadewi,, .- and Syahriar Nur Maulana Malik Ibrahim,, .- and Ana Mariatul Khiftiyah,, .- and Khudrotul Nisa Indriyasari,, .- and Tri Nurhariyati,, .- and Tini Surtiningsih, .- and Hanif Yuliani,, .- and Ni’matuzahroh (2020) Corn Cob Hydrolyzate From Penicillium Citrinum H9 As An Alternative Substrate For Bio Surfactant Production By Hydrocarbonoclastic Bacteria. Ecology, Environment and Conservation, 26 (2020). S169-S175. ISSN 0971-765X

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Abstract

Corn cob is a potential agricultural waste that can be utilized as raw materials in bioindustries. Commonly, corn cob is regarded as agricultural waste and would be eliminated by burning, however this elimination method would produce a toxic fume that polluted the atmosphere. So, new utilization methods of corn cob need to be developed. This research aimed to reveal the potency of corn cob hydrolyzate (CCH) that produced enzymatically using Penicillium citrinum H9 as substrate for biosurfactant production. The CCH was used as growth substrate for biosurfactant production by seven indigenous bacteria of Balongan oil sludge. All isolates were tested the growth and the capability of biosurfactant production through surface tension and emulsification activity measurement. One potential isolate was obtained from this observation. BP1(5) isolate has a lowering surface tension at 56.64±4.63 mN/m in five days incubation and the growth rate was 0.52 “cell/hour. The best biosurfactant product was obtained at concentration 200 ppm of CCH in five days incubation by 23.93±3.56 mN/m in surface tension reductions but the emulsification activity was not detected. This research showed that the CCH be utilized as substrate for biosurfactant production by hydrocarbonoclastic bacteria.

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