Fatimah*, .- and Suharjono, .- and Tri Ardyati,, .- and Ni’matuzahroh, .- and Afaf Baktir, .- and Ahmad Thontowi, .- (2016) Identification and Characterization of Biosurfactant Producing Bacteria Arthrobacter sp. P2(1). Journal of Pure and Applied Microbiology (JPAM), 10 (1). pp. 151-156. ISSN ISSN: 0973-7510 E-ISSN: 2581-690X

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Abstract

The aims of this research were to identify and characterize bacteria isolated from petroleum contaminated soil, P2(1) isolate. This isolate was able to grow on glucose containing medium and produce biosurfactant. This isolate was identified by morphological, physiological and biochemical characteristics and phylogenetic analysis by 16S rDNA sequences. Morphological characteristics observed were colony and cell morphology. Physiological characteristics were observed by microbact kit 12A, 12B. Phylogenetic tree was constructed by comparing 16S rDNA sequences. The results showed that P2(1) isolate was classified into Genus Arthrobacter based on some characteristics, Gram negative in young culture and Gram positive in old culture, rod coccus cycle, and has cream colony on Nutrient Agar medium. The phylogenetic analysis of 16S rDNA sequences showed that P2(1) isolate has similarity with Arthrobacter sp. XBGRY2 (HQ891968.1) with 95% similarity. It can be concluded that the isolate was identified as Arthrobacter sp. P2(1) (KU361211).

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