Ni’matuzahroh and Intan Ayu Pratiwi,, .- and Tini Surtiningsih, .- and Fatimah,, .- and Sri Sumarsih, .- (2017) The potency of Micrococcus sp. L II 61 bacteria as oil sludge cleaning agent. Berkala Penelitian Hayati (Journal of Biological Researches), 22 (2). pp. 38-42. ISSN ISSN: 08526834 | E-ISSN:2337-389X

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Abstract

This research aimed to reveal the ability of Micrococcus sp. L II 61 bacteria that was isolated from Pegirian Surabaya as oil cleaning agent. This is an experimental research to detect the presence of biosurfactant and lipase enzyme in culture supernatant of Micrococcus sp. L II 61 with aliphatic hy-drocarbon (cooking oil) as a substrate growth. Biosurfactant production was evaluated by measuring the surface tension of supernatant using tensiom-eter du Nouy and measuring the emulsification activity value using diesel oil as hydrocarbon test. Lipase enzyme was detected by measuring lipolitic activity value of crude enzyme (culture supernatant) by using p-nitrofenil palmitic (p-npp) as a substrate test. Data were analyzed descriptively. The results showed that Micrococcus sp. L II 61 produced biosurfactant with surface tension decreasing of culture supernatant up to 30.27 ± 1.17 mN/m compared than aquadest and value of hydrocarbon emulsification activity (AE 1 hour) up to 20.24 ± 0.68 %. Culture of Micrococcus sp. L II 61 after 16 hours incubation have a lipolytic activity 33.53 ± 0.14 U/mL at pH 7 and 37 oC. Supernatant of Micrococcus sp. L II 61 100% (v/v) give the high-est percentage of oil sludge solubility, i.e. 86.38 ± 2.39%. Micrococcus sp. L II 61 is a highly potential to be developed as oil sludge cleaning agent.

Item Type:	Article
Uncontrolled Keywords:	Micrococcus sp. L II 61, biosurfactant, lipase enzyme, oil sludge, cleaning agent
Subjects:	Q Science Q Science > Q Science (General) Q Science > QR Microbiology
Divisions:	08. Fakultas Sains dan Teknologi > Program Studi Biologi (S2)
Creators:	Creators NIM Ni’matuzahroh UNSPECIFIED Intan Ayu Pratiwi,, .- UNSPECIFIED Tini Surtiningsih, .- UNSPECIFIED Fatimah,, .- UNSPECIFIED Sri Sumarsih, .- UNSPECIFIED
Depositing User:	Mr Vega Andi Budiman
Date Deposited:	08 May 2023 01:04
Last Modified:	04 Jun 2023 23:16
URI:	http://repository.unair.ac.id/id/eprint/125867
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