Ni’matuzahroh*, .- and Trikurniadewi N, .- and Pramadita A. R. A, .- and Pratiwi I. A, .- and Salamun, .- and Fatimah, .- and Sumarsih Sri, .- (2016) Biodegradation of naphthalenn and phenanthren by Bacillus subtilis 3KP. In: PROCEEDING OF INTERNATIONAL BIOLOGY CONFERENCE 2016: Biodiversity and Biotechnology for Human Welfare. AIP Publishing.

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Abstract

The purposes of this research were to know growth response, degradation ability, and uptake mechanism of naphthalene and phenanthrene by Bacillus subtilis 3KP. Bacillus subtilis 3KP was grown on Mineral Synthetic (MS) medium with addition of 1% yeast extract and naphthalene and phenanthrene respectively 200 ppm in different cultures. Bacillus subtilis 3KP growth response was monitored by Total Plate Count (TPC) method, the degradation ability was monitored by UV-Vis spectrophotometer, and the uptake mechanism of hydrocarbon was monitored by emulsification activity, decrease of surface tension, and activity of Bacterial Adherence to Hydrocarbon (BATH). Bacillus subtilis 3KP was able to grow and show biphasic growth pattern on both of substrates. Naphthalene and phenanthrene were used as a carbon source for Bacillus subtilis 3KP growth that indicated by the reduction of substrate concomitant with the growth. At room temperature conditions (± 30°C) and 90 rpm of agitation for 7 days, Bacillus subtilis 3KP could degrade naphthalene in the amount of 70.5% and phenanthrene in the amount of 24.8%. Based on the analysis of UV-Vis spectrophotometer, three metabolites, 1-hydroxy-2-naphthoic acid, salicylic acid, and pyrocatechol were found in both cultures. The metabolite identification became basis of propose degradation pathway of naphthalene and phenanthrene by Bacillus subtilis 3KP. The results of hydrocarbon uptake mechanism test show that Bacillus subtilis 3KP used all of the mechanism to degrade naphthalene and phenanthrene.

Item Type:	Book Section
Subjects:	Q Science Q Science > Q Science (General) Q Science > QR Microbiology Q Science > QR Microbiology > QR1-502 Microbiology
Divisions:	08. Fakultas Sains dan Teknologi > Biologi
Creators:	Creators NIM Ni’matuzahroh*, .- UNSPECIFIED Trikurniadewi N, .- UNSPECIFIED Pramadita A. R. A, .- UNSPECIFIED Pratiwi I. A, .- UNSPECIFIED Salamun, .- UNSPECIFIED Fatimah, .- UNSPECIFIED Sumarsih Sri, .- UNSPECIFIED
Depositing User:	Mr Vega Andi Budiman
Date Deposited:	22 Jun 2023 03:34
Last Modified:	22 Jun 2023 03:34
URI:	http://repository.unair.ac.id/id/eprint/125905
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