Interaction and cellular uptake of surface-modified carbon dot nanoparticles by J774. 1 macrophages

Lester Thoo, .- and Mochamad Zakki Fahmi, .- and Ihsan N. Zulkipli, .- and Natasha Keasberry, .- and Adi Idris., .- (2017) Interaction and cellular uptake of surface-modified carbon dot nanoparticles by J774. 1 macrophages. Central-European Journal of Immunology, 42 (3). pp. 324-330. ISSN 1426-3912 ; 1644-4124

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Carbon dot (Cdot) nanoparticles are an emerging class of carbon nanomaterials with a promising potential for drug delivery and bio imaging applications. Although the interaction between Cdots and non-immune cell types has been well studied, Cdot interactions with macrophages have not been investigated. Exposure of Cdot nanoparticles to J774.1 cells, a murine macrophage cell line, resulted in minimal toxicity, where notable toxicity was only seen with Cdot concentrations higher than 0.5 mg/ml. Flow cytometric analysis revealed that Cdots prepared from citric acid were internalized at significantly higher levels by macrophages compared with those prepared from bamboo leaves. Interestingly, macrophages preferentially took up phenylboronic acid (PB)-modified nanoparticles. By fluorescence microscopy, strong blue light-specific punctate Cdot fluorescence resembling Cdot structures in the cytosolic space was mostly observed in J774.1 macrophages exposed to PB-modified nanoparticles and not unmodified Cdot nanoparticles. PB binds to sialic acid residues that are overexpressed on diseased cell surfaces. Our findings demonstrate that PB-conjugated Cdots can be taken up by macrophages with low toxicity and high efficiency. These modified Cdots can be used to deliver drugs to suppress or eliminate aberrant immune cells such as macrophages associated with tumors such as tumor-associated macrophages.

Item Type: Article
Uncontrolled Keywords: carbon dots, macrophage, nanoparticles, phenylboronic acid, J7741 cells
Subjects: Q Science
Q Science > Q Science (General)
Q Science > QD Chemistry
Q Science > QD Chemistry > QD450-801 Physical and theoretical chemistry
Divisions: 08. Fakultas Sains dan Teknologi > Kimia
Lester Thoo, .-UNSPECIFIED
Mochamad Zakki Fahmi, .-UNSPECIFIED
Ihsan N. Zulkipli, .-UNSPECIFIED
Natasha Keasberry, .-UNSPECIFIED
Depositing User: Mr Vega Andi Budiman
Date Deposited: 12 Jun 2023 00:18
Last Modified: 12 Jun 2023 00:18
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