Miratul Khasanah, .- and Handoko Darmokusumo, .- and Ganden Supriyanto, .- and Ahmad Zaky Pulungana, .- and Putut Satrio Dahono, .- (2015) The Influence of Ascorbic Acid, Creatinine and Urea on the Analysis of Uric Acid in the Blood Serum by Stripping Voltammetry using Graphite Electrode. In: Proceedings of The 9 th Joint Conference on Chemistry. Chemistry Department, FSM, Diponegoro University 2015, Diponegoro University 2015. ISBN 978-602-285-049-6

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Abstract

The voltammetry method using bare electrode to analyse uric acid encountered a major problem concerning the interference from other organic compound which present together in the serum sample. This research studied the effect of ascorbic acid, creatinine and urea in the analysis of uric acid by stripping voltammetry using graphite electrodes. The deposition potential of uric acid on the surface of electrode was 0.3 V during 60 s at the pH 5. The analytical performance of the method were as follows: precision (RSD) of 0.17%-0.89% for concentrations of 0.1 g/L – 0.5 g/L, sensitivity of 1.331 μA.L/g, detection limit of 0.036 g/L, and accuracy of 97.0 % -105.6%. The creatinine was found not to interfere the uric acid analysis, but urea and ascorbic acid significantly interfere on the uric acid analysis by this method. Analysis of uric acid in the serum sample showed lower result as compared to that done by spectrophotometric method, with recovery of 88%.

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