Medhi Denisa Alinda, - and Muhammad Yulianto Listiawan, - and Anang Endaryanto, - and Heri Suroto, - and Evelyn Komaratih, Evelyn and Fedik Abdul Rantam, - and Bagus Haryo Kusumaputra, - and Regitta Indira Agusni, - and Yuyun Rindiastuti, - and Camilla Amanda Prakoeswa, - and Cita Rosita Sigit Prakoeswa, - Beneficial Effect of Resveratrol on Conditioned Medium Human Adipose-derived Mesenchymal Stem Cell for Cutaneous Wound Healing Related Growth Factors. Malaysian Journal of Medicine and Health Sciences, 17. pp. 11-16. ISSN eISSN 2636-9346

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Abstract

Abstract Introduction: Cutaneous wound healing, a type of soft tissue injury, needs the interaction between multiple growth factors (epidermal growth factor [EGF], transforming growth factor [TGF], platelet-derived growth factor [PDGF], and vascular endothelial growth factor [VEGF]) in order to attain physiological healing. Nevertheless, in certain conditions, i.e., chronic wound, certain growth factors might be insufficient. VEGF is among the most significant pro-angiogenic mediators during wound healing to increase migration and invasion of endothelial cells. Conditioned medium adipose-derived mesenchymal stem cells (CM-AdMSCs) are a new hope to ameliorate impaired healing. Resveratrol (RV), a small molecule that can increase the effect of CM-AdMSCs, is needed to help stimulates growth factors from stem cells for culture. This study aims to evaluate growth factors in CM-AdMSCs with resveratrol. Methods: Isolated and characterized MSCs from CM-AdMSCs treated with RV supplemented Serum Deprived Medium (SDM) were used in this study. Growth factors level were measured using ELISA. Results: A decrease of mean were noted in EGF (290.19±53.19 VS 271.64±52.58) and VEGF (788.76±204.82 VS 117.92±49.22), while an increase of mean in TGF (633.14±11.38 VS 824.73±121.15) and PDGF (2999.00±203.54 VS 3961.40±378.68) between CM-AdMSCs without resveratrol and with resveratrol group, respectively. PDGF and VEGF were statistically significant (p < 0.05). Conclusion: In conclusion, RV promotes PDGF secretion and decreases VEGF in CM-AdMSCs.

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