Fedik Abdul Rantam, - and Cita Rosita Sigit Prakoeswa, - and Damayanti Tinduh, - and Aryati, - (2021) Characterization of SARS-CoV-2 East Java isolate, Indonesia. F1000Research, 10. p. 17. ISSN 20461402

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Abstract

Background: Incidents of SARS-CoV-2 in East Java increased steadily, and it became the second epicenter in Indonesia. The COVID-19 pandemic caused a dire multisectoral crisis all around the world. This study investigates and characterizes local isolates from East Java, Indonesia. Methods: There were 54 patients suspected with SARS-COV-2 infection and 27 patients were COVID-19 positive. Virus isolates were obtained from COVID-19 inpatients’ nasopharyngeal swabs at the Dr Soetomo Teaching Hospital, Surabaya. There were only three isolates (#6, #11, #35) with good growth characteristics. Serial blind passage and cytopathic effect observation in the Vero E6 cell line were performed for virus isolation. Confirmation of the SARS-CoV-2 infection was proven by means of reverse transcriptase-polymerase chain reactions using SARS-CoV-2 specific primers, scanning electron microscopy, and scanning transmission electron microscopy examination. Whole genome sequencing was performed using ARTIC protocol. Furthermore, SARS-CoV-2 characterization was identified through a western blot using rabbit serum immunized with inactive SARS-CoV-2 vaccine and human natural COVID-19 infection serum.

Item Type:	Article
Uncontrolled Keywords:	Based on molecular characterization and immunogenicity of SARS-CoV-2 East Java, Indonesia showed high titer and it has mutation in some regions
Subjects:	R Medicine > RC Internal medicine > RC109-216 Infectious and parasitic diseases
Divisions:	01. Fakultas Kedokteran > Patologi Klinik
Creators:	Creators NIM Fedik Abdul Rantam, - NIDN0010035907 Cita Rosita Sigit Prakoeswa, - NIDN8865610016 Damayanti Tinduh, - NIDN8889900016 Aryati, - NIDN0015086304
Depositing User:	Unnamed user with email indah.fatma@staf.unair.ac.id
Date Deposited:	09 Jun 2023 03:49
Last Modified:	09 Jun 2023 03:49
URI:	http://repository.unair.ac.id/id/eprint/127077
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