Folic Acid Supplementation in Pregnant Mice: An Approach to reduce the Expression of TNF-A and Placental Apoptosis Index in Maternal Stress

Ivon Diah Wittiarika, - and Agus Sulistyono, - and Budi Prasetyo, Budi and Lunardhi Susanto, - and Rize Budi Amalia, - and Erni Rosita Dewi, - Folic Acid Supplementation in Pregnant Mice: An Approach to reduce the Expression of TNF-A and Placental Apoptosis Index in Maternal Stress. Research Journal of Pharmacy and Technology, 15 (8). ISSN 0974-3618

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Official URL: https://rjptonline.org/AbstractView.aspx?PID=2022-...

Abstract

ABSTRACT: Introduction: Maternal stress is harmful to the placenta. It induces apoptosis. Maternal folic acid (FA) supplementation is mandatory in Indonesia and other countries. FA is known as an antioxidant and probably reduces the apoptosis index in placenta with maternal stress. Aim: To investigate the folic acid supplementation in pregnant stress mice (Mus Musculus) on the apoptosis index and placental TNFa expression. Methods: Twenty-one Mus musculus strainded balb/c female mice were randomly divided into three groups. The first group was normal mice, the second group was given stress, and the third group was given stress and folic acid supplementation (3 mg/kg body weight/day in 0,5ml suspension). The ovulation was induced by pregnant mare's serum gonadotropin and HCG. The stress was given by immobilizing the mice in a transparent pipe and giving bright light twice a day on gestational day 10-15. On gestational day 16, the mice were sacrificed, and the placenta tissue was taken. The expression of TNF-a and apoptotic index was calculated by multiplying the score for % stained cells and staining intensity. Result: The expression of TNF-a (mean±SD) of the normal, second, and third groups were 2.1±0.37, 5.9±1.01, and 3.2±1.78. The apoptotic index (mean±SD) of the normal, second, and third groups were 2.7±1.09, 6.3±0.87, and 3.2±0.65. Conclusion: Folic acid supplementation reduces TNF-a and apoptotic index expression in the placenta with maternal stress, and there is a positive correlation between TNF-a and apoptotic index.

Item Type: Article
Subjects: R Medicine > R Medicine (General) > R5-920 Medicine (General)
Divisions: 01. Fakultas Kedokteran > Obstetri Dan Ginekologi (Sub Spesialis)
Creators:
CreatorsNIM
Ivon Diah Wittiarika, -UNSPECIFIED
Agus Sulistyono, -UNSPECIFIED
Budi Prasetyo, BudiNIDN0003057605
Lunardhi Susanto, -UNSPECIFIED
Rize Budi Amalia, -UNSPECIFIED
Erni Rosita Dewi, -UNSPECIFIED
Depositing User: arys fk
Date Deposited: 13 Jun 2023 07:36
Last Modified: 13 Jun 2023 07:36
URI: http://repository.unair.ac.id/id/eprint/127312
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