Polymerase chain reaction of human cytomegalovirus from liver and urine compared with serological test in cholestasis infants

Alphania Rahniayu, - and Gondo Mastutik, - and Anny Setijo Rahaju, - and Siti Eriaty Nur Ruslan, - and Priangga Adi Wiratama, - and Erna Sulistiyani, - and Bagus Setyoboedi, Bagus Polymerase chain reaction of human cytomegalovirus from liver and urine compared with serological test in cholestasis infants. Journal of Infection in Developing Countries, 16 (10). pp. 1630-1636. ISSN 1972-2680 E-ISSN:2036-6590

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Official URL: https://jidc.org/index.php/journal/article/view/36...

Abstract

Abstract Introduction: The most common infection in cholestatic infants is caused by human cytomegalovirus (HCMV). The aims were to detect the presentation of HCMV in cholestatic infants and to evaluate the concordance, sensitivity, and specificity between serology and polymerase chain reaction (PCR) of HCMV from liver biopsy and urine specimens. Methodology: A descriptive observational study with a cross-sectional approach was conducted on 35 cholestatic infants with ethical approval. Specimens were liver biopsy, urine, and anti-HCMV serology. Liver and urine specimens were performed to nested PCR, followed by statistical analysis. Results: PCR from the liver biopsy and urine specimen were positive in 74.3% and 85.7%, respectively. There was no concordance between IgM with the liver PCR, but there was a concordance between IgM with the urine PCR and between IgG with the liver and urine PCR. The sensitivity and specificity of IgM with the liver PCR were 46 % and 56%, respectively, with a diagnostic accuracy of 49%. While IgG sensitivity was 96% with a diagnostic accuracy of 80%. IgG sensitivity and IgM specificity compared with the urine PCR were 93% and 100%, respectively, with a diagnostic accuracy of more than 60%. Conclusions: It demonstrates a high prevalence of HCMV DNA in urine and liver biopsy from cholestatic infants. HCMV PCR assay is more sensitive and specific than the anti-HCMV IgM, but IgG has high sensitivity and accuracy diagnostic. Therefore, serological examination is an option for diagnosing HCMV infection in cholestatic infants in developing countries with no PCR facilities.

Item Type: Article
Subjects: R Medicine > R Medicine (General) > R5-920 Medicine (General)
Divisions: 01. Fakultas Kedokteran > Ilmu Kesehatan Anak (Sub Spesialis)
Creators:
CreatorsNIM
Alphania Rahniayu, -UNSPECIFIED
Gondo Mastutik, -UNSPECIFIED
Anny Setijo Rahaju, -UNSPECIFIED
Siti Eriaty Nur Ruslan, -UNSPECIFIED
Priangga Adi Wiratama, -UNSPECIFIED
Erna Sulistiyani, -UNSPECIFIED
Bagus Setyoboedi, BagusNIDN8889700016
Depositing User: arys fk
Date Deposited: 10 Jul 2023 00:49
Last Modified: 17 Oct 2023 04:17
URI: http://repository.unair.ac.id/id/eprint/127770
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