Dwi Wahyu Indriati, Dwi Wahyu Indriati and Adiana Mmutamsari Witaningrum, Adiana Mmutamsari Witaningrum and M. Qushai Yunifiar, M. Qushai Yunifiar and Siti Qmariyah Khairunisa, Siti Qmariyah Khairunisa and Shuhei Ueda, Shuhei Ueda and Tomohiro Kotaki, Tomohiro Kotaki and Nasronudin, Nasronudin and Masanori Kameoka, Masanori Kameoka The Dominance of CRF01_AE and the Emergence of Drug Resistance Mutations Among Antiretroviral Therapy-Experienced, HIV-1-infected Individuals in Medan, Indonesia. Acta Medica Indonesiana, 52 (4). pp. 366-374. ISSN 0125-9326

	Text (Validasi & Kesesuaian Bidang Ilmu) The Dominance of CRF01_AE and the Emergence of Drug Resistance Mutations Among Antiretroviral Therapy.pdf Download (569kB)
	Text (Artikel) The Dominance of CRF01_AE and the Emergence of Drug Resistance Mutations Among Antiretroviral.pdf Download (1MB)
	Text (Turnitin) The Dominance of CRF01_AE and the Emergence of Drug Resistance Mutations.pdf Download (2MB)
	Text (Bukti Koresponding) The Dominance of CRF01_AE and the Emergence of Drug Resistance Mutations.pdf Download (2MB)

Abstract

human immunodefciency virus type 1 (HIV-1) infection is a serious public health threat worldwide. Medan is one example of big cities in Indonesia with a high prevalence of HIV-1 infection; however, quite a limited study had conducted for detecting the circulation of HIV-1 subtypes in Medan. In addition, a serious factor that can implicate the treatment of HIV-1-infected individuals is the emergence of drug resistance mutations. Thus, the information on HIV-1 infection is important to improve the treatment for infected individuals. Methods: sixty-seven antiretroviral therapy-experienced, HIV-1-infected individuals were recruited for this study. HIV-1 pol genes encoding protease (PR genes) and reverse transcriptase (RT gene), as well as env and gag genes, were amplifed from DNA derived from peripheral blood samples. HIV-1 subtyping was conducted to study the dominant HIV-1 subtype circulating in the region. In addition, the emergence of drug resistance mutations was analyzed based on the guidelines published by the International Antiviral Society-United States of America (IAS-USA). Results: the dominant HIV-1 subtype found in Medan was CRF01_AE (77.6%). In addition, another subtype and recombinant viruses such as recombinants between CRF01_AE and subtype B (12.2%), subtype B (4.1%), and CRF02_AG (4.1%) were also found. Drug resistance-associated major mutations were found in 21.6% (8/37) of RT genes and 3.1% (1/32) of PR genes studied. Conclusion: our study showed that the dominant subtype found in ART-experienced, HIV-1-infected individuals residing in Medan was CRF01_AE. The emergence of drug resistance mutations in RT and PR genes indicated the importance to monitor the prevalence of drug resistance mutations among HIV-1-infected individuals in Medan.

Actions (login required)

View Item