Fitria Ardhiani (2012) Karakterisasi Molekuler Gen Penyandi OMP 2a Brucella Abortus Isolat Lokal. Thesis thesis, Universitas Airlangga.

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Abstract

This study was aimed to know characterization of gene coding OMP 2a B. abortus local isolate through nucleotide analysis by PCR and sequencing, to analyze the homology B. abortus local isolate and isolate from another country including B. abortus isolate strain 19, and to know the profile of outer membrane protei with SDS PAGE method. The sample bacteries were isolated from infected cattle from Sulawesi and NTT. The presence of omp2a genes which are common to all Brucella abortus samples can be confirmed and amplified using Illustram PureTaq Ready-To-Go¬PCR Beads from Invitrogen with one pairs of specifically-designed primers. The product was purificated using Q1Aquick spin column from Qiagen and sequenced using Abi Prism 310 Genetic Analyser— Applied Biosystems to get the omp2a gene nucleotide data The result of this study showed that the isolates have a high homology of omp2a gene with another bacteries isolate from some country and B. abortus isolate strain 19. The result of SDS PAGE is band protein with moleculer weight from Sulawesi were 98 kDa, 86 kDa, 69 kDa, 47 kDa, 43 kDa, 36 kDa, and from NTT were 98 kDa, 86 kDa, 65 kDa , 47 kDa , 43 kDa , 36 kDa.

Item Type:	Thesis (Thesis)
Additional Information:	KKC KK TKH Fit k
Uncontrolled Keywords:	Brucella abortus, outer membrane protein, omp 2a, moleculer characterization
Subjects:	S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > SF910 Other diseases and conditions
Divisions:	06. Fakultas Kedokteran Hewan > Ilmu Penyakit dan Kesehatan Masyarakat Veteriner
Creators:	Creators NIM Fitria Ardhiani 060942010
Contributors:	Contribution Name NIDN / NIDK Thesis advisor Nunuk Dyah Retno Lastuti, Prof. Dr., drh., M.S. UNSPECIFIED Thesis advisor Suwarno, Dr., drh., M.Si. UNSPECIFIED
Depositing User:	Sulistiorini
Date Deposited:	11 Jan 2024 03:36
Last Modified:	11 Jan 2024 03:39
URI:	http://repository.unair.ac.id/id/eprint/129112
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