Deka Uli Fahrodi (2014) Analisis Imunogenisitas Virus Dengue Inaktif (Denv-I, Denv-2, Denv-3, Derv-4) pada Mencit (Mus Musculus) sebagai Kandidat Vaksin Koktail Dengue. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Dengue virus (DENV) is a single stranded RNA virus that circulates in the body of primate and causes DHF for human. Dengue virus have four serotypes (DENV-l, DENV-2, DENV-3, DENV-4). Four dengue virus serotypes do have a similar, yet distinct antigenic properties. Infection only one serotype will give immunity to one serotype is concerned, but does not provide cross- immunity. This study aims to determine the immunogenicity of dengue virus (DENV-l, DENV-2, DENV-3 and DENV-4) inactive as a candidate dengue cocktail vaccine. This study used 18 mices were divided into three treatment groups, PO immunized 0.5 cc PBS, PI immunized with cocktail of dengue virus (DENV-l, DENV-2, DENV-3 and DENV-4) inactive as much as 0.5 cc TCIDsol07 , P2 immunized cocktail of dengue virus (DENV-l, DENV-2, DENV-3 and DENV-4) inactive as much as 0.5 cc TCIDso108. At day 0,7,14 and 21 days of 18 mice that had been treated blood drawn to determine the levels of immunoglobulin M and G with indirect elisa method. On day 28, the treatment group PI and P2 in the challenge test with a cocktail immunized with dengue virus (DENV-l, DENV-2, DENV-3 and DENV -4) inactive 0.5 cc TCIDso108 for the presence of viremia. The results of this study are significant differences (p<0.05) between the groups PO (control), PI and P2 in terms of inducing titers of immunoglobulin (Ig M, Ig G, Ig GIA, Ig G2a and Ig G2B ) . In the challenge test using inactivated dengue virus (DENV-l, DENV-2, DENV-3 and DENV-4) TCIDsol08 in experimental animals (PI and P2), then the PCR test followed by electrophoresis results showed the absence of viremia or viral dengue as evidenced by a negative PCR result.

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