Ratna Sari Yudaningrum (2015) Pengaruh Lactoferria Terhadap Pembentukan Biofilm Klebsiella Pneumoniae Yang Diisolasi Dari Kasus Mastitis Pada Sapi Perah. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Mastitis is the inflammation of the mammary gland and is extremely problematic in the dairy industry. Klebsiella pneumoniae is one of the main infectious agents responsible for causing mastitis. Though not as common as E. coli, Klebsiella's economic impact may be more devastating. The response of Klebsiella mastitis to treatment is poor. Many cows die or end up being culled because of chronic high cell counts. We speculate that one of the reasons behind the prevalence of K. pneumoniae infections is its ability to form biofilms. The objectives of this study were to determine in vitro biofilm formation and the presence of extracelluler matrix associated with biofilm production, we also observed the effect lactoferrin in the biofilm production. Tweleve strains of Klebsiella pneumoniae isolated from chronic mastitic cows, were tested by microtiter plate (MP) and Phase Contrast Microscopy (PCM). The MP methods were used to observed the effect of lactoferrin (LF) in the biofilm production. Klebsiella pneumoniae ATCC13833, was used as a control in bacterial identification and biofilm formation. The LF were used in three different concentration 0.1 mg/ml (LFO.1), 0.5 mg/ml (LFO.5) dan 0.85 mg/ml (LFO.85). There are 12 strain displayed a biofilm-phenotype when tested by MP including the A TCC and one strain not forming biofilm. The strains were further classified as wcak (41.67 %) and strong (50%) biofilm tormers. LFO.l is enhanced the forming of biofilm in vitro as for LFO.5 and LFO;S5 diminished biofilm formation of K. pneumoniae in vitro.

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