Deteksi Dini Toksoplasmosis Pada Otak Mencit (Mus Musculus) Dengan Teknik Polymerase Chain Reaction (Pcr)

Irtya Sofh Anggia (2024) Deteksi Dini Toksoplasmosis Pada Otak Mencit (Mus Musculus) Dengan Teknik Polymerase Chain Reaction (Pcr). Skripsi thesis, Universitas Airlangga.

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DETEKSI DINI TOKSOPLASMOSIS PADA OTAK MENCIT (MUS MUSCULUS) DENGAN TEKNIK POLYMERASE.pdf

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Official URL: http://lib.unair.ac.id

Abstract

Toxoplasmosis is a zoonotic disease caused by intracellular parasite Toxoplasma gondii. Toxoplasmosis related to the presence of toxoplasmosis encephalitis that is often caused of death in people with AIDS. This research was aimed to determine the presence of beginning period T. gondii in the brain of mice by Polymerase Chain Reaction (PCR). Polymerase chain reaction has been extensively used for diagnosis recently because of its very high sensitivity and specificity. Eighteen of mice Balb/C were used in the research, each mouse was infected intraperitoneally with lx103 RH strain of T. gondii tachyzoites. Brain of mice samples were collected once every 12 hour. Brain samples were kept in -20°C, extraced DNA and then examined by PCR. In the process used PCR primers of specific T. gondii SAG-1. The PCR products were analyzed by electrophoresis. The data of the PCR product was analyzed descriptively. The result of the research showed that PCR 806 bp for band length indicated of T. gondii in the brain of mice. The result show that the presence of takizoit detected in the brain of mice on day 9 after infection.

Item Type: Thesis (Skripsi)
Uncontrolled Keywords: Toxoplasmosis, brain, Polymerase Chain Reaction, SAG-1, early detection
Subjects: S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine
Divisions: 06. Fakultas Kedokteran Hewan > Ilmu Kedokteran Hewan Dasar
Creators:
CreatorsNIM
Irtya Sofh AnggiaNIM060911216
Contributors:
ContributionNameNIDN / NIDK
ContributorMufasirinNIDN0011076705
ContributorLaba MahaputraNIDN0024115202
Depositing User: Dwi Marina
Date Deposited: 22 Feb 2024 06:23
Last Modified: 22 Feb 2024 06:23
URI: http://repository.unair.ac.id/id/eprint/130142
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