KHORLIFIY ANAIDILLAH (2015) DETEKSI PENULARAN Toxoplasma gondii PADA FETUS MENCIT (Mus musculus) MOLAi UMUR KEBUNTINGAN 8,5 HARi PADA INDUK MEN CITY ANG DI INFEKSI SECARA INTRA VAGINA DENGAN METODE Polymerase Chain Reaction (PCR) PENELITIAN EKSPLORATIF LABORATORIS. Thesis thesis, Universitas Airlangga.

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Abstract

Toxoplasmosis is a disease caused by the protozoa called Toxoplasma gondii. This disease is zoonotic and generally asymptomatic. Toxoplasmosis can attack various species of mammals, pets, birds, animals, and also humans. Toxoplasmosis transmission is congenital infection from mother to fetus through the placenta. This study aimed to determine whether there is transmission of T. gondii and when the case of T. gondii infection in mice fetuses start of gestation 8.5 days on the parent, infected intravaginal through examination of amniotic fluid and fetal liver of mice using Polymerase Chain Reaction (PCR). This study used 24 pregnant mice at 8.5 day gestation infected intravaginal with dose 10 T.gondii tackyzoite each mice, one mice were sacrificed every 12 hours. Polymerase Chain Reaction (PCR) process using SAG-I primer of T. gondii. Results from the study indicate amniotic fluid fetal mice with gestation 18,5, 19,5 and 20,5 days infected by T. gondii (positive), while in the fetal liver of mice not infected with T. gondii (negative).

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