Hani Plumeriastuti, - and Djoko Legowo, - and Arimbi, - and Nam-Yong Park, - (2009) Karakterisasi Molekul Determinan Pada Proses Apoptosis Sebagai Dasar Pengenalan Pola Patogenesis Penyakit Gumboro. Other thesis, UNIVERSITAS AIRLANGGA.

Text Dr HANI PLUMERIASTUTI DRH M KES.pdf Download (13MB)

Abstract

The main purpo~e of thj~J study was to discover the tfi@eha~j~m tJf ehjeken bursa apoptosis increasing in infeetiOY5 bursal disease virus infectiort, Forty-four ~chicke~ on the first step of experiment are devided inw 2 groups. Chickens in Group I were infected with 1000 TCID 50 of Ta§ik '98 f!iclat@ if11~etieus bursal disease virus through intraocular, peroral, and intracloacal routes. Chickens in group D were treated with physiological NaCl trough intraocular, peroral, and intracloacal routes M placebo. Randomly, each two chicken from 2 groups, were sacrificed on 12, 14, 16, 18, 20, 22, 24, 48, 72, 96, 120 day post infection. The bursa was collected for observation of Fas, ~~lnzyme, caspase 3 expressions and the number of apoptotic cells. Tunell Assay with Apoptotic kit Apoptag was used to observe bursal apoptosis and immunohitochernistry was used to observe the·expression ofFas, granzyme, and caspase 3. The result showed that the 25 % increasing of Fas, granzyme, and caspase 3 expressions and apoptotic cells number were found on 48 hours post infection. It used for determination for Sacrificing chicken in the second step of experiment. Sixteen chickens on the second step of experiment are devided into 2 groups. Chickens in Group I were infected with 1000 TCID 50 of Tasik '98 isolate infectious bursal disease virus through intraocular, peroral, and intracloacal routes. Chickens in group ll were treated with physiological NaCl by intraocular, peroral, and intracloacru rout~~ as placebo. Fourty-eight hours post infection, all of the chicken were sacrificed. The bursa was collected for observation in Fas, granzyme, caspase 3 expressions and the number of apoptotic cells. Tunell Assay with Apoptotic kit Apoptag was used to observe bursal apoptosis and immunohistochemistry was used to observe the expression of Fas, gra.nzyme. and caspase 3. The data were analysed by hotelling's T and path analysis. The result showed that the expression of Fas, granzyme, a.nd ~pa§e 3, and the number of apoptotic cells in bursa were significant difference (p < 0 . 0~) ootw~~n infected chicken and non .. infected chicken. All variables (Fas, granzyme, and ca5f}Mf;i 3 ~pr~ssicm and apoptotic cells nw-nber ) were increasing in infected chickens. The path-rumly~e showed that there was positive correlation between Fas expression and apoptotic bursa cells number in infected chickens. This study suggested that mechanism of bursa apoptosis in infection of infectious bursal disease virus trough Fas-Fas ligand pathway.

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