Dentira Dewi Yusvarianty, D (2015) Pembuktian Gen Penyandi Resisten Antibiotik Tetrasiklin (tetA) dan Sulfonamida (suU) pada Escherichia Coli Yang Diisolasi dari Susu Segar di Surabaya. Thesis thesis, UNIVERSITAS AIRLANGGA.
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Abstract
Escherichia coli is a bacteria that normally grow in the digestive tract, but in certain circumstances may be pathogens that cause gastrointestinal diseases in both humans and animals with clinical symptoms of diarrhea. These bacteria are also capable of contaminating food or beverage in which Escherichia coli is a bacteria that often contaminate milk. Escherichia coli infection performed an act of treatment with antibiotics, but the use of antibiotics is making a problem of bacterial resistance to antibiotics that may occur in the genetic transfer. Resistances to tetracyclines and sulfonamides among Escherichia coli isolates are the most prevalent. In this study explains that the gene coding for tetracycline (tetA) and sulfonamide (sull) antibiotic resistance of Escherichia coli isolated from fresh milk in Surabaya. 40 samples of milk in the milk can there were 13 positive samples contained Esherichia coli that had been planted in the EMBA media and was confirmed by Indol test. These isolates were identified as Escherichia coli tested for antibiotic tetracycline and sulfonamide resistance by the diffuse disc method. The tetracycline and sulfonamide resistant isolates then conducted confirmatory test by Polymerase Chain Reaction using a primer tetA and sull. The result of this research showed lot of 12 positive samples contained bacteria Escherichia coli and resistant to tetracyclines there were 11 positive samples showed the presence of bands DNA primer amplification product tetA with a length of 178 bp and 11 samples of Escherichia coli bacteria that were resistant to sulfonamides there are only five samples positive the band su/J DNA primer with a length of 160 bp.
| Item Type: | Thesis (Thesis) | |||||||||
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| Uncontrolled Keywords: | Esherichia coli, antibiotic resistance, tetracycline, sulfonamide, Polymerase Chain Reaction. | |||||||||
| Subjects: | S Agriculture > SF Animal culture > SF191-275 Cattle S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > Including veterinary genetics, ethology, anatomy, physiology, embryology, pathology |
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| Divisions: | 06. Fakultas Kedokteran Hewan > Ilmu Penyakit dan Kesehatan Masyarakat Veteriner | |||||||||
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| Depositing User: | indah rachma cahyani | |||||||||
| Date Deposited: | 19 Mar 2024 01:28 | |||||||||
| Last Modified: | 19 Mar 2024 01:28 | |||||||||
| URI: | http://repository.unair.ac.id/id/eprint/131847 | |||||||||
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