Astari Puteri, - and Gwenny Ichsan Prabowo, - and Marjono Dwi Wibowo, Marjono and Willy Sandhika, - and Retno Handajani, - (2022) Association Analysis of TP53 Mutations in Codon R175 And G245 With Histopathological Grading Outcomes in OSCC Patients. International Journal of Scientific Advances, 3 (3). ISSN '2708-7972

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Abstract

Abstract Oral squamous cell carcinoma (OSCC) is a malignant tumor lesion in the oral cavity area with less than 50% five-year survival rate for decades. Mutation of the TP53 gene in codons R175 and G245 is a reliable genetic diagnostic for prognosis and prediction of therapeutic response. However, the association of these mutations with the histopathological grading of the OSCC is still unknown. This study aimed to see the association between TP53 mutations in codon R175 and G245 with histopathological grading outcomes in OSCC patients. Forty-four FFPE samples were stained with hematoxylin-eosin to observe their histopathological grade. The mutation was tested through the PCR-RFLP method using Hha1 and Aci1 enzymes. Data recorded were analyzed using Cramer’s V association. This study showed 43.2% of heterozygous mutations in codon R175 and 4.5% of heterozygous mutations in codon G245. Very strong significant associations were found between the histopathological grading result and the mutational status of both TP53 codons R175 (V=0.631; p=0.000) and codons G245 (V=0.303; p=0.044). To be concluded, there was a very strong significant association between the mutational status of TP53 on both codons R175 and G245 with the histopathological grading outcome in OSCC patients.

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