Isdwiranto Iskanto (2004) Peran SOD, H 20 2 dan MDA pada Peningkatan Enzim Kreatin Fosfokinase di Cairan Serebrospinalis Setelah Cedera Kepala Berat. Disertasi thesis, UNIVERSITAS AIRLANGGA.

	Text KKB KK Dis Isk p_ ABSTRACT.pdf Download (845kB)
	Text (FULLTEXT) KKB KK Dis Isk p.pdf Restricted to Registered users only Download (7MB) | Request a copy

Abstract

Recently much evidence has been reported that reactive oxygen species could be one of the major mechanisms in the primary and secondary damage of traumatic brain injury. Most of this evidence is experimental, while clinical data are more rare. The primary and secondary mechanisms of traumatic brain injury may lead to tissue death with the resultant release of the cellular enzyme creatine kinase into the cerebrospinal fluid. In order to investigate the reactive oxygen species after severe head injury and study the correlation with the brain enzyme creatine kinase, we have performed this study. The first purpose of this study is to analyze the level of reactive oxygen species and creatine kinase in the CSF after severe head injury and its correlation with the severity of trauma. The second purpose is to clarifY the relationship between reactive oxygen species and creatine kinase after severe head injury. Using a protocol approved by our institutional review board, 18 cerebrospinal fluid samples from 7 patients with severe head injury (Glasgow Coma Scale score < or = 8) and 5 controls were studied. Cerebrospinal fluid was drained as standard care after severe head injury. CSF was assessed on day 1,3, and 5 after ventricular drain placement. Biochemical markers of reactive oxygen species included antioxidant superoxide dismutase, hydrogen peroxide, and malondialdehyde. Creatine kinase after severe head injury was markedly increased versus control on day I (86,00 ± 53,39 UII versus 4.4 ± 2.79 UII, P = 0.0035). Antioxidant superoxide dismutase was reduced versus control (10.033 ± 2.073 Il / ml versus 12.213 ± 0.22 Il / ml., p = 0.016). Hydrogen peroxide was markedly reduced (34,17 ± 14,52 CPM versus 74,71 ± 16,44 CPM, P =0,0005), and malondialdehyde was markedly increased versus control (192.97 ± 77.5 nmol/ml versus 18.04 ± 3.39 nmol/ml, p =0,0005). The result showed a correlation between the reactive oxygen species with creatine kinase in the CSF of patients with severe head injury and a correlation between reactive oxygen species and creatine kinase with the severity of trauma (p < 0.05). The results also showed that CSF biochemical markers measurement may help both from the point of view of diagnostics and individualization of antioxidants administration, and also these markers could be used to monitor antioxidant strategies in clinical trials.

Actions (login required)

View Item