ENNY YULIANI, - (2000) PEMISAHAN SPERMATOZOA DENGAN METODE SWIM UP DAN KOMBINASI SWIM UP DENGAN ASIDE MIGRATION PENGARUHNYA TERHADAP RASIO KROMOSOM SEKS (Studi Eksperimental Untuk Menghasilkan Embrio Sapi Bali Jantan). Disertasi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Purposes : To evaluate the effectiveness and the mechanism of Swim up (SU method) and Swim up + Aside migration (SU + ASM) combination method on spermatozoa quality (motility, live spermatozoa and normal morphology) and spermatozoa fertilizing ability (in vitro fertilization rate and embryo cleavage rate), also an increasing of Y -chromosome bearing spermatozoa ratio of Bali Cattle based on spermatology paradigm with the concept of time, motility and in vitro fertilization. Methods : The type of this research was laboratoric true experimental with treatments using SU method (30, 45 and 60 minutes) and SU + ASM combination ( 30 minutes SU and 30 minutes ASM) on a number of samples devided into three groups. These groups consisted were one control group (K) and two treatment group (group -1 and -11). Independent variable in this research were SU (30, 45 and 60 minutes) and SU + ASM combination (30 minutes SU with 30 minutes ASM). Dependent variable comprised to (a) sperm quality (motility, live spermatozoa and normal morphology); (b) in vitro fertilization, (c) in vitro embryo cleavage rate and (d) Y- chromosome bearing spermatozoa ratio based on head size and chromosomal analysis. Intervening variable was the oocyte maturation. Results : The result showed a significant difference by using Manova test (p~0,05) in sperm motility, live spermatozoa and normal morphology amongs treatment groups and control group. SU 30 treatment group established the highest normal sperm morphology, compared with the other groups. The result of Anova test revealed a significant difference (p~0,05) in fertilization rate between treatment group and control group. SU 30 and SU 45 treatment group was significantly higher, when compared to SU 60 and SU+ASM combination groups. From Anova analysis, it was found that there was a significant difference (ps0,05) in embryo cleavage between treatment groups and control group. SU 30 and SU + ASM treatment group was significantly higher (ps0,05) compared with control, SU 45 and SU 60 groups, at the level of embryo development observed 48 hours after fertilization. It was showed that in all of the treatment groups, embryos developed ~ 4 cells were significantly higher (p~0,05) than embryos developed > 4 cells, whereas no any difference was found between quantity of blastomer cells in the control group. According to Anova analysis, it was showed that Y-chromosome bearing spermatozoa in treatment group were significantly different (psO,CS), compared with control groups. Y chromosome bearing spermatozoa ratio of SU + ASM treatment group was significantly higher (ps0,05) if compared with SU 30, SU 45 and SU 60 groups. xviii IR - PERPUSTAKAAN UNIVERSITAS AIRLANGGA DISERTASI PEMISAHAN SPERMATOZOA... ENNY YULIANI Conclusion : Treatment with SU 30 method was able to increase the motility, the live spermatozoa and the normal morphology of Bali cattle spermatozoa. Preparation by using SU and SU + ASM combination gave a positive effect on in vitro fertilization rate in Bali catlle, and the higher fertilization rate was resulted from SU 30 and SU 45 ·treatment group. SU + ASM combination could increase in vitro embryo cleavage rate in Bali catlle and was "very effective" for increasing Y- chromosome bearing sperm ratio in Bali catlle. Suggestion : Based on these results had been obtained by using several techniques above, it was therefore, SU or the combination of both techniques should be able at least to increase male Bali cattle population through Artificial Insemination or Embryo transfer technique.

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