Retno Wulan Randayani, - (2010) Potensi Antibodi Anti-Neuraminidase Asal Kuning Telur (IgY) Sebagai Upaya Imunoterapi Terhadap Infeksi Virus Avian Influenza : Penelitian Eksplorasi Laboratoris. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Neuraminidase (NA) is the major surface glycoprotein of the avian influenza virus which processes critical enzymatic activity and has been considered as a suitable target for designing agents against influenza viruses. NA protein can induce antibody inhibitor of NA virus activity in replication cycle in host cell. The purpose of this study is to prove anti-NA antibody of egg yolk (IgY) inhibition of growth of Avian Influenza virus subtype HSNI with inhibition hydrolysis of galactose and N-asetylneuraminic acid of oligosacharidaglycoprotein chain. This study represents of laboratories exploration and descriptive research. This study is devided become three phase; first, characterization of neuraminidase protein with SDSPAGE and western Blot Methods, Elusi of neuraminidase protein, determination of protein rate result of elusi, immunogenic and antigenic test of neuraminidase protein. Second, Isolation of antibody anti-NA egg yolk, purifieatiaR annoNA antibody, immUnagerue teat Of anti-NA antibody using dot blot method. Third, Challenge and immunotherapy at chickens, detection antibody-idiotype (Anti-anti-NA) and immunohistochemistry method for detection Avian Influenza virus and tying anti-NA antibody in tropism cell. The Result of this study, chickens with doses of immunotherapy using anti-NA antibody 50, 100 and 200 Ilg given before infection, at the same times with infection, one day after infection and two days after infection showed percentage of protection successively 93,3 %, 53,3 %, 73,3 % dan 6,7 %. The result of immunohistochemistry showed that antigen of Avian Influenza virus were detected in ileum of chicken with doses 0 Ilg, 50 Ilg and 100 NA protein can induce antibody and can reaction with anyi-NA antibody specificly; Antibody of anti-NA have ability fasten antigen and can protect chicken againts avian influenza virus subtype HSNI infection up to 93,3%; mechanism protection againts Avian Influenza subtype HSNI infection happened with inhibition hydrolysis of galactose and Nasetyineuraminic acid of oligosacharida-glycoprotein chain by ~i-NA antibody at cell tropism; and anti-idiotype antibody (anti-anti-NA) not detected at chickens.

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