Prof. Sri Agus SudJarwo.,Ph.D., drh, - and Prof. Dr. Fedik Abdul Rantam, drh, - and Drh. Koerniasarl.,M.Kes, - (2010) PRODUKSI DAN PURIFIKASI IMUNOGLOBULIN Y ANTI DENGUE YANG BERPOTENSI SEBAGAI IMUNOTERAPI PADA DEMAM BERDARAH. Other thesis, UNIVERSITAS AIRLANGGA.

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Abstract

"Immunoglobulin Y (1gY) in yolk has been shown in several studies to prevent both bacterial and viral infections. This research. was conducted to find evidence that IgY specific against dengue virus can be produced in a large quantity in egg yolk. Laying hens were vaccinated with active-vaccine (Institute Tropical Disease). The IgY was purified using al pity chromatograpy technique, and antidengue activity was measured using agar gel immunodifusion (AGID). The concentration of IgY was calculated., and the protein pattern was detected using polyacrilarnid gel electrophoresis (SDS-PAGE). Antibody of dengue was detected and produce aspecific line of precipitation in AGID. The concentration of IgY was 115 mgiml. Purified specific IgY consist protein bands with molecular weights ranging from 50 to 75 kD. These results indicated the possibility of using specific IgY for passive immunisation to prevent dengue infection or as immunotherapeutic applications for dengue treatment in humans."

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