Fitriah Ardiani, - (2004) Uji Aktivitas Antikanker Ektrak Eter Dan Ekstrak Metanol Tumbuhan Lumut Hati (Marchantia cataractarum Schiffn.) Terhadap Kultur Sel Mieloma Mencit Dengan Metode Viabilitas Sel. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

A research has been done to determine the anticancer activity of the aether and methanol extracts of livewort (Marchantia cataractarum Schiffn.). The anticancer activity was determined on the myeloma cell culture using cell viability test which is based on the cell resistance ability against cytotoxic materials. Trypan blue 0,4 % staining is used to differ the morphology between the death and live cells. The death ones would be stained blue while the live remain unstained. The myeloma cell were incubated in RPMI media contained 10 % of Fetal Bovine Serum (FBS). The concentration of aether and methanol extracts of Marchantia catarctarum Schiffn. were 1000 ppm, 100 ppm, 10 ppm and 1 ppm. Etoposide was used as possitive control in three different concentration (100 ppm, 10 ppm and 1 ppm) while RPMI 1640 was used as negative control. Their solution were added into myeloma cell culture in microwell plate and then incubated for 24 hours, at 37oC in CO2 incubator. The extracts effect on the myeloma cell culture was examined to find out the number of viable myeloma cells using a hemocytometer. The cell viability data were analyzed statistically using one way annova and probit. The result on the myeloma cell culture by cell viability test indicated that aether and methanol extracts of Marchantia cataractarum Schiffn significantly have anticancer activity with LC50 of aether extract was 68,32 ppm and LC50 of methanol extract was 92,24 ppm. Futher characterization and isolation are still needed for development in the future.

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