Indrawati, Dwi Wahyu and Setiawatie, Ernie Maduratna and Rahayu, Retno Pudji and Syah Manurung, Rizky Briliant and Aljunaid, Mohammed Ahmed (2026) Exploring the bioactive potential of bovine pericardium membrane combined with hyaluronic Acid: characterization and cellular viability analyses. Biochemistry and Biophysics Reports, 45.

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Abstract

This study explores the bioactive potential of bovine pericardium membranes combined with hyaluronic acid (HA, 120 kDa) at concentrations of .5, 1.0, and 2.0 through physicochemical characterization and fibroblast cell viability analysis. A laboratory experimental design was employed to evaluate bovine pericardium membranes modified with hyaluronic acid at different concentrations. Material characterization was conducted employing Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and X-ray Diffraction (XRD) analyses. Fibroblast cell viability was assessed using the MTT assay, and statistical analysis was conducted using the ANOVA test. The results demonstrated that hyaluronic acid modification altered the physicochemical characteristics of the membrane, including increased surface hydrophilicity. The cell viability test revealed comparable fibroblast viability among HA-modified membranes, indicating no cytotoxic effects. FTIR and SEM analyses confirmed chemical interactions and morphological features associated with favorable cell�material interactions. These findings suggest that bovine pericardium membrane with hyaluronic acid 120 kDa exhibit favorable physicochemical characteristics and in vitro cytocompatibility, indicating their potential for tissue engineering applications. This research provides a scientific foundation for developing innovative biomaterials that support favorable cell�material interactions for tissue regeneration research, contributing to advancements in tissue regeneration therapy. © 2026 The Authors.

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