Nur Aini Mulyadi, - (2017) Pengembangan Formula Liposom Kering Ovalbumin Menggunakan Lioprotektan Sukrosa dan Matriks Pendispersi HPMC (Sebagai Sistem Penghantaran Vaksin). Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Disaccharides, such as sucrose, have been reported to protect phospholipid membranes during drying, while HPMC is used as gel matrix to inhibit recrystallization. We have investigated the solid state characterization of dried liposome formulation (lyophilized) which was preserved in the presence of sucrose and dispersed in HPMC matrix. The dried liposomal formulation featured cationic dioctadecyldimethylammoniumbromide (DODAB) was produced to overcome detrimental phase separation in phospholipid membranes during production process. The effect of sucrose and HPMC on lipid phase behavior during dehydration were characterized including thermodynamic properties such as morphology by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), thermal behaviour of lipid bilayer by differential scanning calorimetry (DSC), crystallinity by powder x-ray diffractometry (PXRD), and abilty to protect ovalbumin which was seen with ovalbumin band by SDS-PAGE and quantification of ovalbumin by Bradford assay. Data revealed that the presence of sucrose and HPMC in the formula FS1ova showed a miscible mixture with a broad endothermic peak at 104.13°C, relatively less crystalline-forming properties, and the percentage decrease in ovalbumin level is equal to 37.65%, which is smaller than other formula, so that potentially constructs a stable dried liposomal. The present study revealed prospective advantages of using combination of sucrose and HPMC in development of dried–DODAB liposomal formulation.

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