WIWIEK INDRIYANI MASKOEP (2019) MEKANISME HAMBATAN lgY SPESIFIK ANTI HIV-1 TERHADAP INFEKSI HIV-1 PADA FASE BINDING ANTARA GP120 DENGAN RESEPTOR CD4, CCR5 DAN CXCR4 LIMFOSIT T CD4+ SECARA IN VITRO. Disertasi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Background : HIV is a cytopathic virus causing HIVIAJDS infections: HIV-1 (which infects the whole world individuals) and HIV-2. Vaccine for HIV infection has not been found and antiretrovirals are only able to suppress infection. An immunotherapy using immunoglobulin-containing ingredients for treating patients with HIVIAIDS patients should be found. The successful use of JgY (Rotamix IgY orally for the treatment of diarrhea in children, H5N1, and Influenza B) has opened wider opportunities for the use of anti-HJV-1 specific Ig Y obtained from Lohmann laying hens yolks immunized with the inactivated HIV-1 virus as passive immunotherapy. Methods: This was a true experimental research to reveal the neutralization activity of anti-HJV-1 specific IgYusing syncytium formation test method, and the density of receptor CD4, CCR5 and CXCR4 were then examined. The research design was post-test only control group. Results: The formation of the Syncytium molecule showed that anti-HJV-1 specific IgY was effective for inhibiting the formation of Syncytium in HIV-1 infections against CD4+ T lymphocytes in the binding stage (entry stage) at 24-hour observation and 7th day (p <0.05). The results of CD4 receptor density using immunohistochemistry method revealed that anti-HJV-1 specific IgY was effective in inhibiting HIV-1 infection against CD4+ T lymphocytes at the binding stage in the treatment group at 24 hour and 7th day observations. The same method done to the CCR5 revealed that anti-HIV-1 specific IgY could inhibit HIV-1 infection against CD4+ T lymphocytes in the binding stage, but did not show significant effectiveness (p-value> 0.05) at 24-hour observation, whereas on the 7th day observation anti-HJV-1 specific IgY was effective in inhibiting HIV-1 infection against CD4+ T lymphocytes in the binding stage (p-value <0.05). Examination of CXCR4 receptor density revealed that anti HIV-1 specific IgY did not show effectiveness in inhibiting HIV-1 infection against CD4+ T lymphocytes in the binding stage at 24-hour observation (p-value> 0.05 ), whereas on the 7th day observation anti-HIV-1 specific IgY was effective in inhibiting HIV-1 infection against CD4+ T lymphocytes in the binding stage (p-value <0.05). Conclusion: Anti-HIV-1 specific IgY was effective in inhibiting HIV-1 infection against CD4+ T lymphocytes in the binding stage (entry stage).

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