Firdausy Kurnia Maulana, - (2018) Analisis Homologi Dan Prediksi Epitop Gen Penyandi 37 Kda Outer Membrane Protein H (Omph) Pasteurella Multocida Tipe B Isolat Lokal Asal Nusa Tenggara Timur (Ntt) : Penelitian Eksploratif Laboratoris. Masters thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Haemorrhagic Septicaemia (HS), in Indonesia known as Ngorok disease is a fatal acute septicemia disease in cattle and buffalo caused by Pasteurella multocida. Outer membrane protein H (OmpH) is one of the major proteins in P. multocida envelope has been purified and characterized as porin immunodominant. OmpH becomes one of the most important bacterial antigens and promising candidates for the development of HS vaccines and diagnostic kits. The aim of this study is to find out the homology of nucleotide sequence of 37 kDa outer membrane protein H (OmpH) gene of P. multocida of local isolate from NTT with vaccine strain (Katha strain) and 5 referen isolates and predict epitope of 37 kDa outer membrane protein H (OmpH) gene P. multocida of local isolate from NTT. Detection of 37 kDa OmpH gene of P. multocida from NTT and vaccine strain was done using PCR and then sequenced to obtain nucleotide sequence. Homology analysis of nucleotide sequences and amino acid sequences was performed on the BLAST program at NCBI. Epitope prediction analysis of 37 kDa OmpH genes P. multocida from NTT and vaccine strains was performed using the online programme B Cell Epitope Prediction Tools. The results showed that the 37 kDa OmpH gene of P. multocida from NTT has 99% homology to vaccine strain (Katha) and isolate from China, Iran, India, Mesir, and 89% homology to isolat from USA and 37 kDa OmpH gene of P. multocida local isolate from NTT were likely to be immunogen candidates based on B cell epitope that had 11 epitopes. These findings indicate that P. multocida local isolate from NTT has a chance as immunogen candidate, which can be developed as a vaccine candidate and diagnostic kit for HS.

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