AULIA INTAN FIRDAUS (2019) EFEK SCAFFOLD BOVINE HYDROXYAPATITE-GELATIN-ALENDRONATE TERHADAP PENUTUPAN DEFECT TULANG DAN EKSPRESI ALKALI FOSFATASE (ALP) PADA MODEL HEWAN OSTEOPOROSIS. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The aim of this study was to analyze the effects of Bovine Hydroxyapatite-Gelatin-Alendronate (BHA-Gel-Ale) scaffold on the closure of bone defect and alkaline phosphatase expression (ALP) in ovariectomy (OVX)-induced osteoporosis in rats. In this study, 48 female Wistar strain rats were divided into 4 groups: negative control (non OVX), positive control (OVX), BHA-Gel (OVX) and BHA-Gel-Ale (OVX). Eight weeks after OVX, a hole was made in the right femur of the rat with a diameter 2.2 mm in all groups and scaffold was given in BHA-Gel and BHA-Gel-Ale groups. The process of closing the defect was observed at 2nd and 6th weeks after the treatment. Analysis was carried out through radiological observation and alkaline phosphatase expression (ALP). Based on the results of the radiological analysis, improvement of bone defect closure in the negative control group were faster than positive control groups. In the BHA-Gel group, improvement of bone defect closure were faster than BHA-Gel-Ale group. The analysis of alkaline phosphatase expression at 2nd week showed that positive control group had significant increase compared to negative control group (p <0.05). But at 6th week, there were no significant difference (p> 0.05). Furthermore, the BHA-Gel group showed higher expression of ALP compared to BHA-Gel-Ale group at 6th week (p <0.05) but not at 2nd week (p> 0.05). In this study, BHA-Gel-Ale scaffold was able to increase the expression of alkaline phosphatase at 6th weeks after the treatment, but radiologically it was unable to accelerate the closure of bone defect in osteoporosis animals models.

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