Mira Novia (2003) PERBANDINGAN METODE SIMPAN BEKU KONVENSIONAL: DENGAN VITRIFIKASI PADA SPERMA PRIA MORMOZOOSPERMIA TERHADAP MORFOLOGI, MOTILITAS, VIABILITAS DAN KONDENSASI KROMATIN SPERMATOZOA. Thesis thesis, Universitas Airlangga.

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Abstract

Comparative study between conventional and vitrification methods on man with normozoospermic sperm towards morphology, motility, viability and chromatin condensation of spermatozoa". (under guidance of Prof. DR. H. Soehartojo Hardjopranjoto, Drh., MSc. and dr. Aucky Hinting Ph.D.). Vitrification method is an alternative for cryopreservation of spermatozoa which is continuously studied and developed because the technique is more efficient, cheaper, simpler and easily carried out, compared to the conventional method. The purpose of this study is to observe the results of the vitrification as well as to examine the quality of spermatozoa after vitrification, so that it can be used as a model for cryopreservation of sperm especially for the ART (Assisted Reproductive Techniques). The study applies cryoprotectants glycerol 15% + HEPES + Human Serum Albumin (Medium Freeze, Fertipro, Belgi). The sample is normozoospermic sperm - 10 ejaculates treated in three ways. The first is observed before cryopreservation, and the other two groups are put in mini straw 0,25 mi., then preserved in frozen condition using conventional and vitrification methods. After being frozen for 24 hours, the sperm are thawed, then stained and observed under microscope. The parameter is the percentage of normal morphology, motility (a+b), viability and chromatin condensation of spermatozoa.

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