VINKA NOVIA YULIANA, - (2022) Uji Toksisitas Akut dan Subkronis Ekstrak Etanol 70% Daun Genitri (Elaeocarpus serratus L.) Secara In Vivo. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Elaeocarpus serratus. L (ES) has known as herbal medicine in Indonesia that currently was proven to be active as antiosteoporosis. In order to develop standardized herbal medicine, acute and subchronic toxicity of 70% ethanol extract of ES leaves were carried out to fulfill the qualification of phytopharmaceutical products registration. For acute toxicity test, a single dose of 5, 50, 300, 2000, and 10000 mg/kg BW of 70% ethanol extract of ES leaves were given orally to male mice. The death and toxicity symptoms were observed for 14 days. For the subchronic toxicity test, doses used were 200, 400, and 600 mg/kg BW of 70% ethanol extract of ES leaves were given orally to male-female Wistar rats for 90 days. The clinical parameters such as hematology, clinical chemistry, relative organ weight, and histopathology organ were observed after 90 days. Their parameters were analyzed statistically by using One-Way ANOVA and Kruskal-Wallis method with a confidence interval of 95% and post-hoc. The study found there were no death and toxicity symptoms found in all mice, so that can be determined LD50 of the extract is more than 10000 mg/kg BW, which is practically nontoxic. On subchronic toxicity test, after clinical symptoms observed such as hematology, clinical chemistry, relative organ weight, and histopathology organ was done and statistically analyzed, the toxic effect showed on male and female rats. It could be concluded that administration of 70% ethanol extract of ES leaves did not cause acute toxic effect on mice and caused a subchronic toxic effect on Wistar rats.

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