ARY SUSANTI, 060213020 (2007) DAYA ANTIBAKTERI EKSTRAK ETANOL DAUN BELUNTAS (Pluchea indica less) TERHADAP Escherichia coli SECARA IN VITRO. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The aim of this study was to investigate the antibacterial activity of the ethanol extract Pluchea indica less leaves against Escherichia coil by in vitro. The method using broth dilution test was determined Minimum Bactericidal Concentration (MBC) with a broth extract cultures into Eosin Methylen Blue Agar (EMBA) medium. The twice tubes of concentration extract of 25% and 50% was showed no visible turbidity, and then inoculated into Eosin Methylen Blue Agar medium. After 24 h of incubation at 37 °C, Minimum Bactericidal Concentration (MBC) was determined which no viable growth of Escherichia coli at Eosin Methylen Blue Agar (EMBA) medium. The result showed that Minimum Bactericidal Concentration (MBC) of the extract of 25% and 50% had been similar (X2<0.05) on the growth of Escherichia coli. Based on this result, Minimum Bactericidal Concentration (MBC) of the extract was used 25% concentration.

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