ADE RATRI YUSTINA, 020810020 (2012) PENINGKATAN JUMLAH OSTEOKLAS PADA KERADANGAN PERIAPIKAL AKIBAT INDUKSI LIPOPOLISAKARIDA PORPHYROMONAS GINGIVALIS ( eksperimental laboratoris ). Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Backgrounds. The pulp chamber is mostly infected by anaerob gram negative bacteria and one of them is Porphyromonas gingivalis. The cell wall of this bacteria containing lipopolysaccharide (LPS). LPS can penetrate to the periradicular tissue, act as endotoxin in host and cause periradicular inflammation that leading to bone destruction. Bone destruction because of the inflammation process mediated by immune system. This inflammation is mediated by immune system so we can found the cytokin pro-inflammatory infiltrated in the lesion and then stimulate macrophage to release IL-1 and TNF-α for bone resorption. Bone cell has an important role in destruction bone is osteoclast. Osteoclasts are multinucleated, terminally differentiated cells which play an essential role in bone resorption. Binding RANK with RANKL are increasing the osteoclastogenesis process. Purpose. To know how many is the increasing number of osteoclast in periapical tissue induced by LPS. Method. We use laboratory experimental with the post test only control group design. A total of 21 sample was divided into 3 groups. Group A: control group, group B: the teeth were induced by lipopolysaccharide, group C: the teeth only induced by PBS steril. The animals were sacrificed on 3 weeks after the experiment and prepared for histological examination of periradicular tissue response. Results. According to the result, the osteoclast increases significantly in group B when lipopolysaccharide was applied in the pulp chamber. The increasing number of osteoclast is a complex process because of the contribution of TLR4, IL-1 and TNF-α. Conclusion. There was an increasing number of osteoclast in periapical inflammation that induced by lipopolysaccharide.

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