MICHAEL SALOMO C.P, 021111144 (2014) PENINGKATAN JUMLAH SEL MAST pada ULKUS TRAUMATIKUS setelah APLIKASI EKSTRAK TERIPANG EMAS (Stichopus hermanii). (PENELITIAN pada TIKUS WISTAR (Rattus norvergicus) ). Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Background. Oral Traumatic ulcer is a lesion formed by oral mucous tissue damage that caused by trauma. In the process of wound healing, mast cell play a role especially in the inflammatory phases. The gold sea cucumber extract (Stichopus hermanii) containing polyunsaturated fatty acid (PUFA) and glycosaminoglicans (GAGs), synergy between PUFA and GAGs will accelerate wound healing and tissue repair. Objective. The aim of this study is to determine the effect gold sea cucumber extract (Stichopus hermanii) toward total mast cell in wistar rats. Method. 40 Rattus novergicus male, weighing 200-300g and aged 8 weeks,were divided into two categories; day-4 and day-7 categories, also 4 control group; negative control group, 20%, 40%, 80% Stichopus hermanii extract consentration. With heated burnisher no.4, the ulcer were induced in lower lip mucous. Ulcer were formed on day-2 and received golden sea cucumber extract an application of gel. Then, five rats each group euthanized on day-4 and day-7. The ulcer area were removed and fixed for histometric to determined numbered of mast cell by using haematoxylineosin staining. All slides were examined by pathologist under microscope from 200x to1000x. All data would be analyzed by using ANNOVA Result. In total mast cell, it showed significant increase (p<0,05) between 80 % consentrations to negative control group, 20%, 40% concentrations on day-4, while on day-7 there were not significant increase between each group. Conclusion. It can be concluded that gold sea cucumber extract at concentration 80 % on day-4 effective to increase the amount of mast cell.

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