Ainani Dwi Hapsary (2015) Daya Hambat Seng Oksida Ekstrak Allium Sativum Linn. Sebagai Bahan Pulp Capping Terhadap Lactobacillus Acidophilus. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Background: Pulp capping treatment is simple endodontic treatment performed on pulp tissue with a diagnosis of reversible pulpitis. Dominant bacteria in the deep caries are Lactobacillus acidophilus. Zinc oxide eugenol usage in an opened pulp is controversial because it can cause irritation. 100% Allium sativum Linn. extract expected be able used as alternative material of eugenol because contains antibacterial compounds such steroids, alkaloids, flavonoids, essential oils, triterpenoids and allicin. Objective: To determined the effect of the zinc oxide 100% Allium sativum Linn. extract as pulp capping material in inhibited the growth of Lactobacillus acidophilus. Methods: Zinc oxide was mixed with 100% Allium sativum Linn. extract using 2 a ratio of 1: and 2:1, then made a suspension of bacteria Lactobacillus acidophilus which was comparable to a McFarland standard 0,5 of 1.5 x 108 CFU / ml. Sixteen petridish containing nutrient agar media (NA) was divided into 2, 8 first petridish each were divided into 4 areas. Eight petridish others were divided into 3 areas. Lactobacillus acidophilus bacteria grown on petridish containing NA media. Petridish contains planted Lactobacillus acidophilus in NA media and given the appropriate treatment and control groups sample was then incubated at a temperature of 37°C in anaerobic atmosphere for 24 hours. Observations and measurements of bacterial growth inhibition zone. Data were analyzed using one-way ANOVA. Result: The results of the analysis of one-way ANOVA found significant differences in inhibition after administration of zinc oxide 100% Allium sativum Linn. extract with ratio of 1: 1 and 2: 1. Conclusion: Results of this study can be concluded that zinc oxide 100% Allium sativum Linn. extract with a ratio of 1: 1 and 2: 1 can inhibit the growth of bacteria Lactobacillus acidophilus.

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